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dna binding dye sample loading reagent  (fluidigm)


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    fluidigm dna binding dye sample loading reagent
    Dna Binding Dye Sample Loading Reagent, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 265 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+binding+dye+sample+assay+loading+reagent/DNA+Binding+Dye/pm41839303-91-119-125
    Average 95 stars, based on 265 article reviews
    dna binding dye sample loading reagent - by Bioz Stars, 2026-09
    95/100 stars

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    Binding Assay:

    Article Title: Parental fasting effects on offspring immune gene expression, epigenetic patterns, and gut microbiota in a species with male pregnancy (Syngnathus typhle).
    Article Snippet: .. AR TIC LE IN PR ES S For the sample pre-mix, we combined 369.6 uL Ssofast-EvaGreen Supermix with Low ROX (Bio-Rad Laboratories, Hercules, CA, USA) with 37 uL 20x DNA Binding Dye Sample & Assay Loading Reagent (Fluidigm). ..

    Article Title: Parental fasting effects on offspring immune gene expression, epigenetic patterns, and gut microbiota in a species with male pregnancy ( Syngnathus typhle)
    Article Snippet: .. For the sample premix, we combined 369.6 μL of Ssofast-EvaGreen Supermix with Low ROX (Bio-Rad Laboratories, Hercules, CA, USA) and 37 μL of 20 × DNA Binding Dye Sample & Assay Loading Reagent (Fluidigm). ..

    Article Title: Parental fasting effects on offspring immune gene expression and gut microbiota in a species with male pregnancy ( Syngnathus typhle)
    Article Snippet: .. For the sample pre-mix, we combined 369.6 uL Ssofast-EvaGreen Supermix with Low ROX (Bio-Rad Laboratories, Hercules, CA, USA) with 37 uL 20x DNA Binding Dye Sample & Assay Loading Reagent (Fluidigm). ..

    Article Title: Trans‐generational plasticity in response to immune challenge is constrained by heat stress
    Article Snippet: .. For chip loading, a sample mix was prepared by combining 3.5 ml 2× Ssofast‐EvaGreen Supermix with Low ROX (Bio‐Rad Laboratories, Hercules, CA, USA) with 0.35 μl 20 × DNA Binding Dye Sample & Assay Loading Reagent (Fluidigm) and 3.15 μl pre‐amplified PCR products. .. An assay mix was prepared by combining 0.7 μl of 50 μM primer pair mix, 3,5 μl Assay Loading Reagent (Fluidigm) and 3.15 μl low EDTA‐TE buffer.

    Polymerase Chain Reaction:

    Article Title: Trans‐generational plasticity in response to immune challenge is constrained by heat stress
    Article Snippet: .. For chip loading, a sample mix was prepared by combining 3.5 ml 2× Ssofast‐EvaGreen Supermix with Low ROX (Bio‐Rad Laboratories, Hercules, CA, USA) with 0.35 μl 20 × DNA Binding Dye Sample & Assay Loading Reagent (Fluidigm) and 3.15 μl pre‐amplified PCR products. .. An assay mix was prepared by combining 0.7 μl of 50 μM primer pair mix, 3,5 μl Assay Loading Reagent (Fluidigm) and 3.15 μl low EDTA‐TE buffer.



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    H3N1-challenge experiment confirms the exacerbated disease phenotype of RIG-I and RIG-I-RNF135 chickens infected with virulent avian influenza viruses. The generated transgenic chickens were challenged at 28 weeks of age with H3N1 and assessed for different parameters. (A) Viral shedding based on tracheal swabbing and viral RNA load analysis. (B) Probability of survival in the challenged groups. (C) Expression of RIG-I , RNF135 , and influenza-regulated genes in the duodenum, lung, and spleen. (D) Histological assessment of the reproductive tract; WT-MOCK: normal macroscopical and histological appearance of the salpinx; RNF-RIG-I-H3N1: severe atrophy with mild fibrinous peritonitis in the infundibulum (asterisk), severe lymphoplasmacytic salpingitis (arrowheads) (40x), the magnum <t>(20x),</t> and vasculitis in the magnum vessels (200x). (E) Scoring of lesions in the reproductive tract in all challenged groups, starting with the upper row WT-MOCK, WT-H3N1, RIG-I -H3N1, and RIG-I-RNF135 -H3N1; FP, fibrinous peritonitis; LPS, lymphoplasmacytic salpingitis; LPV, lymphoplasmacytic vasculitis; EYP, egg yolk peritonitis. Error bars indicate standard error of mean (SEM); ( ∗ ) indicate statistical differences between groups tested simultaneously ( p < 0.05). Depending on the normal distribution of the data, multiple group comparison was done either with one-way ANOVA or the Independent-Samples Kruskal-Wallis Test. Data indicate the results of H3N1 in vivo experiment, where the dots represent individual chickens analyzed.
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    H3N1-challenge experiment confirms the exacerbated disease phenotype of RIG-I and RIG-I-RNF135 chickens infected with virulent avian influenza viruses. The generated transgenic chickens were challenged at 28 weeks of age with H3N1 and assessed for different parameters. (A) Viral shedding based on tracheal swabbing and viral RNA load analysis. (B) Probability of survival in the challenged groups. (C) Expression of RIG-I , RNF135 , and influenza-regulated genes in the duodenum, lung, and spleen. (D) Histological assessment of the reproductive tract; WT-MOCK: normal macroscopical and histological appearance of the salpinx; RNF-RIG-I-H3N1: severe atrophy with mild fibrinous peritonitis in the infundibulum (asterisk), severe lymphoplasmacytic salpingitis (arrowheads) (40x), the magnum (20x), and vasculitis in the magnum vessels (200x). (E) Scoring of lesions in the reproductive tract in all challenged groups, starting with the upper row WT-MOCK, WT-H3N1, RIG-I -H3N1, and RIG-I-RNF135 -H3N1; FP, fibrinous peritonitis; LPS, lymphoplasmacytic salpingitis; LPV, lymphoplasmacytic vasculitis; EYP, egg yolk peritonitis. Error bars indicate standard error of mean (SEM); ( ∗ ) indicate statistical differences between groups tested simultaneously ( p < 0.05). Depending on the normal distribution of the data, multiple group comparison was done either with one-way ANOVA or the Independent-Samples Kruskal-Wallis Test. Data indicate the results of H3N1 in vivo experiment, where the dots represent individual chickens analyzed.

    Journal: Frontiers in Immunology

    Article Title: Genetic reinstatement of RIG-I in chickens reveals insights into avian immune evolution and influenza interaction

    doi: 10.3389/fimmu.2025.1680791

    Figure Lengend Snippet: H3N1-challenge experiment confirms the exacerbated disease phenotype of RIG-I and RIG-I-RNF135 chickens infected with virulent avian influenza viruses. The generated transgenic chickens were challenged at 28 weeks of age with H3N1 and assessed for different parameters. (A) Viral shedding based on tracheal swabbing and viral RNA load analysis. (B) Probability of survival in the challenged groups. (C) Expression of RIG-I , RNF135 , and influenza-regulated genes in the duodenum, lung, and spleen. (D) Histological assessment of the reproductive tract; WT-MOCK: normal macroscopical and histological appearance of the salpinx; RNF-RIG-I-H3N1: severe atrophy with mild fibrinous peritonitis in the infundibulum (asterisk), severe lymphoplasmacytic salpingitis (arrowheads) (40x), the magnum (20x), and vasculitis in the magnum vessels (200x). (E) Scoring of lesions in the reproductive tract in all challenged groups, starting with the upper row WT-MOCK, WT-H3N1, RIG-I -H3N1, and RIG-I-RNF135 -H3N1; FP, fibrinous peritonitis; LPS, lymphoplasmacytic salpingitis; LPV, lymphoplasmacytic vasculitis; EYP, egg yolk peritonitis. Error bars indicate standard error of mean (SEM); ( ∗ ) indicate statistical differences between groups tested simultaneously ( p < 0.05). Depending on the normal distribution of the data, multiple group comparison was done either with one-way ANOVA or the Independent-Samples Kruskal-Wallis Test. Data indicate the results of H3N1 in vivo experiment, where the dots represent individual chickens analyzed.

    Article Snippet: The reaction was prepared by mixing 2.5 μl TaqMan Gene Expression Master Mix (Applied Biosystems), 0.25 μl 20X DNA Binding Dye Sample Loading Reagent (Fluidigm), 0.25 μl 20X EvaGreen DNA binding dye (Biotum) and 2 μl of preamplified cDNA.

    Techniques: Infection, Generated, Transgenic Assay, Expressing, Comparison, In Vivo